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Show restriction sites and translation
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DNA sequence
in plain or fasta format
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or paste here
Note if you copy and paste sequences here, make sure don't break header lines.
Enzymes to use
enzymes given below
all in the database
all commercially available
Restriction enzymes *
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or paste here
Separate names with space(s), not commas.
Your own enzymes and patterns *
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or paste here
Note: If you supply your own enzymes here, all enzyme input above will be ignored.
Cutting end type *
Don't care (click here to change)
known 5' overhang only
known 3' overhang only
known blunt only
known 5' and 3' overhang only
known 5' and 3' overhang and blunt only
known 5' overhang and blunt only
known 3' overhang and blunt only
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Coding start site or reading frame *
translate the start site codon from code table (default as Met)
What is it, if given above?
Coding start site
Reading frame
Genetic code *
Standard code (click here to change)
The Vertebrate Mitochondrial Code
The Yeast Mitochondrial Code
The Mold, Protozoan, Coelenterate Mitochondrial and Mycoplasma/Spiroplasma Code
The Invertebrate Mitochondrial Code
The Ciliate, Dasycladacean and Hexamita Nuclear Code
The Echinoderm and Flatworm Mitochondrial Code
The Euplotid Nuclear Code
The Bacterial, Archaeal and Plant Plastid Code
The Alternative Yeast Nuclear Code
The Ascidian Mitochondrial Code
The Alternative Flatworm Mitochondrial Code
Chlorophycean Mitochondrial Code
Trematode Mitochondrial Code
Scenedesmus obliquus Mitochondrial Code
Thraustochytrium Mitochondrial Code
Pterobranchia Mitochondrial Code
Candidate Division SR1 and Gracilibacteria Code
The Genetic Codes
Also print protein sequence separately?