The following is an example output of the program.
1 TGGAAAGGAGTGTAGGCTAATTCCTTGCTCTTCCACTTAAACACTATAGTTTCTGACTTG
ACCTTTCCTCACATCCGATTAAGGAACGAGAAGGTGAATTTGTGATATCAAAGACTGAAC
^
44 BdiSI
61 ACCAACTCAGAGGACCCAGGTTCCACCATAGGAGAAGTGGGGAAGGGCTGTCATTCTCTT
TGGTTGAGTCTCCTGGGTCCAAGGTGGTATCCTCTTCACCCCTTCCCGACAGTAAGAGAA
^ ^
72 BcrI BcuAI Bfi105I, 79 BcrI
121 TGGATAGATGTGGGGCCACAAAGTAGCTCAAGGCTGGACCAACATAGCACAGCTTTGTGA
ACCTATCTACACCCCGGTGTTTCATCGAGTTCCGACCTGGTTGTATCGTGTCGAAACACT
^^^ ^
132 BcrI, 133 Bfi105I, 134 BecAII Bfi458I, 156 BcuAI
Bfi105I
181 GCTATTGCCCACCAAATAAGTGAGGGGAAACCTTGACCCTTTTATAATTGGTATGCCCAG
CGATAACGGGTGGTTTATTCACTCCCCTTTGGAACTGGGAAAATATTAACCATACGGGTC
241 CCCTCTGATTATTGACTACCTTCCTTCTTTTAATGTTGCAGAGAATTAAGGAGCTAGAAG
GGGAGACTAATAACTGATGGAAGGAAGAAAATTACAACGTCTCTTAATTCCTCGATCTTC
^
294 BfaI
MetA
301 AAAGAATAGAAACCCAGAAGAGGCAAATAAAGGAGCTGGAAGAAAAGGTAAAACAGATGA
TTTCTTATCTTTGGGTCTTCTCCGTTTATTTCCTCGACCTTCTTTTCCATTTTGTCTACT
snGlyGluArgValCysAsnTrpArgLysGlyThrTrpAlaCysLeuThrSerHisTyrL
361 ATGGGGAGAGGGTGTGCAACTGGAGGAAGGGGACTTGGGCTTGCCTGACATCACATTATA
TACCCCTCTCCCACACGTTGACCTCCTTCCCCTGAACCCGAACGGACTGTAGTGTAATAT
ysAsnProSerSerGlyLeuProValGluValArgProAlaAsnTrpProMetGlnSerA
421 AGAACCCAAGCTCAGGCCTCCCAGTTGAGGTCAGGCCTGCAAATTGGCCCATGCAAAGCA
TCTTGGGTTCGAGTCCGGAGGGTCAACTCCAGTCCGGACGTTTAACCGGGTACGTTTCGT
^ ^ ^
435 BecAII Bfi458I, 454 BecAII Bfi458I, 466 BecAII
Bfi105I Bfi458I
rgValGluThrAsnLysArgGluProValGlyProGluLeuTrpValLeuTrpAlaArgV
481 GAGTGGAGACGAACAAAAGGGAGCCTGTTGGGCCAGAGCTGTGGGTACTCTGGGCCAGAG
CTCACCTCTGCTTGTTTTCCCTCGGACAACCCGGTCTCGACACCCATGAGACCCGGTCTC
^ ^^ ^^
500 BcrI, 510 Bfi105I, 511 BecAII Bfi458I, 532 Bfi105I,
533 BecAII Bfi458I
alArgSerProSerHisSerLeuThrProValAlaLeuAlaLeuProGlnPheProLeuT
541 TCAGGAGCCCCTCACACAGCCTCACCCCCGTAGCCCTTGCACTGCCTCAGTTTCCCCTCT
AGTCCTCGGGGAGTGTGTCGGAGTGGGGGCATCGGGAACGTGACGGAGTCAAAGGGGAGA
^
544 BcrI
rpThrTyrMetLeuAsnGln
601 GGACCTATATGTTGAACCAGTGACCTGGGGTCCTGATCTATGCTGGTGCCCATTGTGGTG
CCTGGATATACAACTTGGTCACTGGACCCCAGGACTAGATACGACCACGGGTAACACCAC
^ ^^ ^ ^
601 BcuAI Bfi105I, 628 BcrI, 629 BcuAI Bfi105I, 635 Bfi57I,
645 BcrI
661 CAGAGGGTTTAACCTTCCAGGTCCATCAACCTTGGCTGTCTGAAGATAAAGTTCCTGAAA
GTCTCCCAAATTGGAAGGTCCAGGTAGTTGGAACCGACAGACTTCTATTTCAAGGACTTT
^
680 BcuAI Bfi105I
721 CACAGGCCATGATCCCCAAGAGGCACAGGAACATTTTGTCCCTGATCCCCAAGAAGTGTC
GTGTCCGGTACTAGGGGTTCTCCGTGTCCTTGTAAAACAGGGACTAGGGGTTCTTCACAG
^ ^ ^
725 BecAII Bfi458I, 731 Bfi57I, 764 Bfi57I
781 TCCAGTACCAGAGATGCTAGGGTGCCTGCTCAAGCACTTTCTCCAGACCCTGACTGCAGC
AGGTCATGGTCTCTACGATCCCACGGACGAGTTCGTGAAAGAGGTCTGGGACTGACGTCG
^ ^ ^
797 BfaI, 801 BcrI, 834 BdiSI
841 CTGGCATGCAGGGCCTCTGGTCACTGGTATCAGCAGACTCAGTGCTCCTCAGACCAAGTT
GACCGTACGTCCCGGAGACCAGTGACCATAGTCGTCTGAGTCACGAGGAGTCTGGTTCAA
^^
851 Bfi105I, 852 BecAII Bfi458I
901 GAAAAGTATGTGCCTAGAGAACCCTGCCCCTTGCAGGTCTCCATGCATTCATTTCAGGGC
CTTTTCATACACGGATCTCTTGGGACGGGGAACGTCCAGAGGTACGTAAGTAAAGTCCCG
^
914 BfaI
961 AGTTCCATATCCTCTGCGATTTGAGGCAGG
TCAAGGTATAGGAGACGCTAAACTCCGTCC
BcrI GGNNCC 8: 72 79 132 500 544 628
645 801
BcuAI G^GWCC 5: 72 156 601 629 680
BdiSI C^TRYAG 2: 44 834
BecAII GG^CC 8: 134 435 454 466 511 533
725 852
BfaI C^TAG 3: 294 797 914
Bfi105I GGNCC 10: 72 133 156 466 510 532
601 629 680 851
Bfi458I GGCC 8: 134 435 454 466 511 533
725 852
Bfi57I ^GATC 3: 635 731 764
Following enzymes don't cut:
BctI BcuI BdaI BdiI BepI BetI
BfaSII
Note: Tst1, Ava2 and Gri1i were not recognized!
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