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Cutting End Type

The cutting end type is checked only for enzymes that cut within or next to their recognition sequences and whose cleavage sites are marked in Rebase. Those enzymes that cut outside their recognition sequences, such as DraRI CAAGNAC(20/18), are excluded. Usually it makes sense to specify the end type only if you want to use or list all the (commercially available) enzymes in the database.

Example enzymes with 5' overhang ends:

AbaI          T^GATCA
AccB1I        G^GYRCC
AeuI          CC^WGG
AjnI          ^CCWGG
AspBHII       RG^GWCCY

Example enzymes with 3' overhang ends:

BfuAII        GCATG^C
HpyCH4I       CATG^
HpyCH4III     ACN^GT
HpyF10VI      GCNNNNN^NNGC
Kaz48kI       RGGNC^CY

Example enzymes with blunt ends:

FalII         CG^CG
MluB2I        TCG^CGA
MslI          CAYNN^NNRTG
MspA1I        CMG^CKG